In this project, one hundred timber core samples of Sapele (Entandrophragma), Rosewood (Dalbergia) and Ebony (Diospyros) trees were taken from across West Africa and Madagascar. The 2H, 13C, and 18O isotopes of the extracted cellulose were determined by using an elemental analyser coupled to an isotope ratio mass spectrometer (EA-IRMS). The study revealed that 2H and 18O of cellulose showed no correlation with 2H and 18O of precipitation water, suggesting the leaf-to-air-vapour pressure difference (VPD), as well as alternative ground water sources may influence the isotopes laid down in cellulose of tropical timber. Additionally, multi-element profiles of the timber samples were determined by inductively coupled plasma mass spectrometry (ICP-MS), to assess the potential for geographical origin discrimination based on the combined profile of stable isotope ratios and multi-element concentrations.